mouse igg isotype matched control ab (Novus Biologicals)

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Novus Biologicals mouse igg isotype matched control ab
Mouse Igg Isotype Matched Control Ab, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+igg+isotype+matched+control+ab/Mouse+IgG+Isotype+Control/pm29386556-64-30-37
Average 90 stars, based on 1 article reviews

mouse igg isotype matched control ab - by Bioz Stars, 2026-11

90/100 stars

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Blocking Assay:

Article Title: Recombinant TB10.4 of Mycobacterium bovis induces cytokine production in RAW264.7 macrophages through activation of the MAPK and NF-κB pathways via TLR2.
Article Snippet: The TB10.4 antigen of Mycobacterium bovis/Mycobacterium tuberculosis induces a strong Th1 CD4+ Tcell response.. Thus, it is currently under intensive study as a possible vaccine candidate.. However, how TB10.4 activates innate immune cells is unclear.

Article Title: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2.
Article Snippet: LPS (E. coli 0111:B4) was purchased from Sigma-Aldrich (St. Louis, MO, USA), and Pam3CSK4 was purchased from InvivoGen. .. In experiments designed to block TLR signaling, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse antibody (Ab) against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (all from Imgenex). .. In experiments designed to block NF-κB signaling, RAW264.7 cells were pre-treated for 1 h at 37 °C with 1, 5 or 10 μM NF-κB-specific inhibitor (BAY 11-7082; Sigma-Aldrich) prior to rMtb9.8 exposure as described above.

Article Title: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2
Article Snippet: LPS ( E. coli 0111:B4) was purchased from Sigma-Aldrich (St. Louis, MO, USA), and Pam 3 CSK 4 was purchased from InvivoGen. .. In experiments designed to block TLR signaling, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse antibody (Ab) against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (all from Imgenex). .. In experiments designed to block NF-κB signaling, RAW264.7 cells were pre-treated for 1 h at 37 °C with 1, 5 or 10 μM NF-κB-specific inhibitor (BAY 11-7082; Sigma-Aldrich) prior to rMtb9.8 exposure as described above.

Control:

Article Title: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2.
Article Snippet: .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex). pNF-κB-Luc expression after rMtb9.8 stimulation was measured using a luciferase assay after transfection with the reporter plasmid, pNF-κB-Luc (0.2 μg/well). .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex).

Activation Assay:

Article Title: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2.
Article Snippet: .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex). pNF-κB-Luc expression after rMtb9.8 stimulation was measured using a luciferase assay after transfection with the reporter plasmid, pNF-κB-Luc (0.2 μg/well). .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex).

Expressing:

Article Title: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2.
Article Snippet: .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex). pNF-κB-Luc expression after rMtb9.8 stimulation was measured using a luciferase assay after transfection with the reporter plasmid, pNF-κB-Luc (0.2 μg/well). .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex).

Luciferase:

Article Title: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2.
Article Snippet: .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex). pNF-κB-Luc expression after rMtb9.8 stimulation was measured using a luciferase assay after transfection with the reporter plasmid, pNF-κB-Luc (0.2 μg/well). .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex).

Transfection:

Article Title: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2.
Article Snippet: .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex). pNF-κB-Luc expression after rMtb9.8 stimulation was measured using a luciferase assay after transfection with the reporter plasmid, pNF-κB-Luc (0.2 μg/well). .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex).

Plasmid Preparation:

Article Title: Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2.
Article Snippet: .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex). pNF-κB-Luc expression after rMtb9.8 stimulation was measured using a luciferase assay after transfection with the reporter plasmid, pNF-κB-Luc (0.2 μg/well). .. In experiments designed to evaluate NF-κB activation by TLR2 or TLR4, RAW264.7 cells were pre-treated for 30 min at 37 °C with 30 μg/ml of a mouse Ab against TLR2, TLR4 or a mouse IgG isotype-matched control Ab (isotype Ab) (all from Imgenex).

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